• Users Online: 133
  • Print this page
  • Email this page
REVIEW ARTICLE
Year : 2020  |  Volume : 22  |  Issue : 4  |  Page : 29-34

Molecular and serological tests for COVID-19


Department of Medical Microbiology, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey

Correspondence Address:
Dr. Melahat Gurbuz
Department of Medical Microbiology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03030 Afyonkarahisar
Turkey
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ejop.ejop_43_20

Rights and Permissions

The outbreak of coronavirus disease 2019 (COVID-19) has become a global public health problem. There is little known for test utilization and diagnosis. Tests for COVID-19 include molecular tests and serological tests. Nucleic acid testing is a gold standard, while serological tests are used in seroprevalence. The tests should be selected according to the time course of the virus and serological response. The targeted genes are several. In our country, real-time polymerase chain reaction kit targeting RNA-dependent RNA polymerase gene fragment is being used. Common sample types are nasopharynx and/or oropharynx swabs. Patients with pneumonia sputum, bronchoalveolar lavage fluid, etc., should be tested. The peak concentrations of viral load reach before day 5, and the virus can be detected until the end of the 1st week after the onset of illness from nasal-pharynx. There are also rapid tests either detect the viral components in nasopharyngeal secretions or antibodies. IgM/IgA and IgG antibody are detectable on day 5 and day 14, respectively, after symptom onset. Enzyme-linked immunosorbent assay kits based on recombinant nucleocapsid protein and spike protein are expected to give more reliable results. Rapid diagnostic tests detecting neither antigen nor antibody is the first choice of the World Health Organization for diagnosis but is recommended to be used for surveillance. Ideal guidance in current circumstances is to confirm cases with available tests by following national recommendations so that we should take action as soon as possible, give appropriate therapy, and determine their contacts for infection prevention.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2180    
    Printed56    
    Emailed0    
    PDF Downloaded211    
    Comments [Add]    
    Cited by others 1    

Recommend this journal